Project

Part:BBa_I3002:Design

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-29)


E. colibrator design 3, Polkadorks (I3410.I3200.E0422.I3510.I3401.I3411.I0460.I3100.I3400.I3300.I340


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 551
    Illegal BglII site found at 942
    Illegal BglII site found at 2407
    Illegal BglII site found at 8513
    Illegal BglII site found at 9366
    Illegal BglII site found at 12150
    Illegal BglII site found at 13306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1834
    Illegal AgeI site found at 1363
    Illegal AgeI site found at 2215
    Illegal AgeI site found at 6431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3344
    Illegal BsaI.rc site found at 4447
    Illegal BsaI.rc site found at 12586
    Illegal SapI site found at 1874
    Illegal SapI.rc site found at 715
    Illegal SapI.rc site found at 9530
    Illegal SapI.rc site found at 9990
    Illegal SapI.rc site found at 12314


Design Notes

The layout of E. colibrator (I3002) is designed to insulate the most sensitive devices, namely those in the switch that store state. The hk022cI switch output (ON) is insulated by appearing first. This output is then attached to the AspA attractant sender and the CFP reporter (I3410.I3200.E0422). The quorum receiver and its control of the lambda cI switch input (OFF) occur next (I3510.I3401) because leaky TIPS through this module will encourage the OFF state dicated by the downstream lambda cI switch output (I3411.I0460). Note that the OFF switch output leads to aiiA synthesis to promote HSL degradation and system reset in this design. The rest of the switch inputs occur downstream because they do not store state and therefore do not need to be as insulated from leaky TIPS. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the other (I3300.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, the quorum sender device (I3500) is last because it is meant to be constitutively on at some basal level, so insulation is not required. Note: Depending on the efficacy of the OmpR promoter, this design can be implemented with any of I3300, I3301, I3302 (variations of Tar-EnvZ devices with different OmpR promoters). The default implementation uses I3300.



Source

References